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Hello Katie,
I am a bit new in sequence data analyses. luckily, I found your tutorial easily replicable on my set of data (18SrRNA).
However, I wasn't able to import your custom functions into my R session and use it.
On the other hand, I am using data import from qiime2. I believe all the data processing I did after importing data into R didn't modify the downstream analyses as described in your tutorial.
Thank you for your support.
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