diff --git a/.gitignore b/.gitignore
new file mode 100644
index 0000000..5b6a065
--- /dev/null
+++ b/.gitignore
@@ -0,0 +1,4 @@
+.Rproj.user
+.Rhistory
+.RData
+.Ruserdata
diff --git a/16SrRNA-hex-tutorial.Rproj b/16SrRNA-hex-tutorial.Rproj
new file mode 100644
index 0000000..8e3c2eb
--- /dev/null
+++ b/16SrRNA-hex-tutorial.Rproj
@@ -0,0 +1,13 @@
+Version: 1.0
+
+RestoreWorkspace: Default
+SaveWorkspace: Default
+AlwaysSaveHistory: Default
+
+EnableCodeIndexing: Yes
+UseSpacesForTab: Yes
+NumSpacesForTab: 2
+Encoding: UTF-8
+
+RnwWeave: Sweave
+LaTeX: pdfLaTeX
diff --git a/README.md b/README.md
index 4f428fc..2d6c4e8 100644
--- a/README.md
+++ b/README.md
@@ -6,6 +6,8 @@ The dataset we will be working are the practice dataset from the [H3ABioNet 16S
 
 The table below contains the metadata associated with the dog stool samples. There are three dogs which are treated with increased percentage of a compound in their diet: 5 different treatments (0-4, representing an increased percentage of a compound in their diet).
 
+working on 16S tutorial using DADA2
+
 Sample | Dog | Treatment | Read counts r1 | Read counts r2
 ------ | --- | --------- | -------------- | -------------- 
 Dog1 | B | 2 | 118343 | 118343