diff --git a/.changeset/relax-accept-header.md b/.changeset/relax-accept-header.md
new file mode 100644
index 000000000..c8aa3705f
--- /dev/null
+++ b/.changeset/relax-accept-header.md
@@ -0,0 +1,5 @@
+---
+"@hono/mcp": patch
+---
+
+Relax Accept header validation in MCP Streamable HTTP transport for broader client compatibility
diff --git a/packages/mcp/src/streamable-http.test.ts b/packages/mcp/src/streamable-http.test.ts
index 30891f319..2aad864c8 100644
--- a/packages/mcp/src/streamable-http.test.ts
+++ b/packages/mcp/src/streamable-http.test.ts
@@ -608,15 +608,15 @@ describe('MCP helper', () => {
     expectErrorResponse(errorData, -32000, /Client must accept text\/event-stream/)
   })
 
-  it('should reject POST requests without proper Accept header', async () => {
+  it('should reject POST requests that explicitly accept neither json nor sse', async () => {
     sessionId = await initializeServer()
 
-    // Try POST without Accept: text/event-stream
+    // Try POST with Accept header that includes neither json nor sse
     const response = await server.request('/', {
       method: 'POST',
       headers: {
         'Content-Type': 'application/json',
-        Accept: 'application/json', // Missing text/event-stream
+        Accept: 'text/plain', // Neither application/json nor text/event-stream
         'mcp-session-id': sessionId,
         'mcp-protocol-version': '2025-03-26',
       },
@@ -628,10 +628,72 @@ describe('MCP helper', () => {
     expectErrorResponse(
       errorData,
       -32000,
-      /Client must accept both application\/json and text\/event-stream/
+      /Client must accept application\/json or text\/event-stream/
     )
   })
 
+  it('should accept POST requests with only application/json Accept header', async () => {
+    sessionId = await initializeServer()
+
+    const response = await sendPostRequest(server, TEST_MESSAGES.toolsList, {
+      'mcp-session-id': sessionId,
+      'mcp-protocol-version': '2025-03-26',
+      Accept: 'application/json',
+    })
+
+    expect(response.status).not.toBe(406)
+  })
+
+  it('should accept POST requests with only text/event-stream Accept header', async () => {
+    sessionId = await initializeServer()
+
+    const response = await server.request('/', {
+      method: 'POST',
+      headers: {
+        'Content-Type': 'application/json',
+        Accept: 'text/event-stream',
+        'mcp-session-id': sessionId,
+        'mcp-protocol-version': '2025-03-26',
+      },
+      body: JSON.stringify(TEST_MESSAGES.toolsList),
+    })
+
+    expect(response.status).not.toBe(406)
+  })
+
+  it('should accept POST requests with wildcard Accept header', async () => {
+    sessionId = await initializeServer()
+
+    const response = await server.request('/', {
+      method: 'POST',
+      headers: {
+        'Content-Type': 'application/json',
+        Accept: '*/*',
+        'mcp-session-id': sessionId,
+        'mcp-protocol-version': '2025-03-26',
+      },
+      body: JSON.stringify(TEST_MESSAGES.toolsList),
+    })
+
+    expect(response.status).not.toBe(406)
+  })
+
+  it('should accept POST requests with no Accept header', async () => {
+    sessionId = await initializeServer()
+
+    const response = await server.request('/', {
+      method: 'POST',
+      headers: {
+        'Content-Type': 'application/json',
+        'mcp-session-id': sessionId,
+        'mcp-protocol-version': '2025-03-26',
+      },
+      body: JSON.stringify(TEST_MESSAGES.toolsList),
+    })
+
+    expect(response.status).not.toBe(406)
+  })
+
   it('should reject unsupported Content-Type', async () => {
     sessionId = await initializeServer()
 
diff --git a/packages/mcp/src/streamable-http.ts b/packages/mcp/src/streamable-http.ts
index b9992f73d..db237f135 100644
--- a/packages/mcp/src/streamable-http.ts
+++ b/packages/mcp/src/streamable-http.ts
@@ -275,19 +275,20 @@ export class StreamableHTTPTransport implements Transport {
   private async handlePostRequest(ctx: Context, parsedBody?: unknown) {
     try {
       // Validate the Accept header
-      const acceptHeader = ctx.req.header('Accept')
-      // The client MUST include an Accept header, listing both application/json and text/event-stream as supported content types.
-      if (
-        !acceptHeader?.includes('application/json') ||
-        !acceptHeader.includes('text/event-stream')
-      ) {
+      const acceptHeader = ctx.req.header('Accept') || '*/*'
+      const acceptsJson =
+        acceptHeader.includes('application/json') || acceptHeader.includes('*/*')
+      const acceptsSse =
+        acceptHeader.includes('text/event-stream') || acceptHeader.includes('*/*')
+
+      if (!acceptsJson && !acceptsSse) {
         throw new HTTPException(406, {
           res: Response.json({
             jsonrpc: '2.0',
             error: {
               code: ErrorCode.ConnectionClosed,
               message:
-                'Not Acceptable: Client must accept both application/json and text/event-stream',
+                'Not Acceptable: Client must accept application/json or text/event-stream',
             },
             id: null,
           }),