stopped in "segmentation Done"

[wyp2010s]

BaxterAlgorithms
Wrote to the settings file of D:\test
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 1 clusters into 5 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Separating 1 clusters into 4 cells using the watershed transform
Separating 1 clusters into 9 cells using the watershed transform
Separating 7 clusters into 25 cells using the watershed transform
Wrote to the settings file of D:\照片素材
Separating 1 clusters into 1180 cells using the watershed transform
Separating 1 clusters into 636 cells using the watershed transform
Separating 1 clusters into 636 cells using the watershed transform
Separating 1 clusters into 636 cells using the watershed transform
Separating 1 clusters into 1180 cells using the watershed transform
Wrote to the settings file of D:\照片素材
Separating 101 clusters into 271 cells using the watershed transform
Separating 101 clusters into 271 cells using the watershed transform
Wrote to file D:\照片素材\Settings.csv
Tracking cells in D:\照片素材\aglz-
Starting parallel pool (parpool) using the 'local' profile ...
connected to 8 workers.
Segmenting image 1 / 100
Segmenting image 2 / 100
Segmenting image 3 / 100
Segmenting image 5 / 100
Segmenting image 8 / 100
Segmenting image 12 / 100
Segmenting image 18 / 100
Segmenting image 25 / 100
Separating 91 clusters into 250 cells using the watershed transform
Separating 105 clusters into 296 cells using the watershed transform
Separating 101 clusters into 271 cells using the watershed transform
Computing features for image 1 / 100
Computing features for image 2 / 100
Separating 88 clusters into 278 cells using the watershed transform
Computing features for image 3 / 100
Separating 102 clusters into 295 cells using the watershed transform
Computing features for image 5 / 100
Separating 87 clusters into 258 cells using the watershed transform
Computing features for image 8 / 100
Computing features for image 12 / 100
Separating 100 clusters into 295 cells using the watershed transform
Computing features for image 18 / 100
Separating 105 clusters into 283 cells using the watershed transform
Computing features for image 25 / 100
Segmenting image 7 / 100
Separating 104 clusters into 286 cells using the watershed transform
Segmenting image 11 / 100
Separating 94 clusters into 260 cells using the watershed transform
Segmenting image 32 / 100
Separating 102 clusters into 287 cells using the watershed transform
Segmenting image 39 / 100
Separating 95 clusters into 288 cells using the watershed transform
Segmenting image 46 / 100
Separating 91 clusters into 267 cells using the watershed transform
Segmenting image 4 / 100
Separating 98 clusters into 280 cells using the watershed transform
Computing features for image 4 / 100
Computing features for image 7 / 100
Computing features for image 11 / 100
Segmenting image 17 / 100
Separating 94 clusters into 279 cells using the watershed transform
Computing features for image 17 / 100
Segmenting image 24 / 100
Separating 85 clusters into 267 cells using the watershed transform
Computing features for image 24 / 100
Computing features for image 32 / 100
Computing features for image 39 / 100
Segmenting image 38 / 100
Computing features for image 46 / 100
Segmenting image 53 / 100
Segmenting image 23 / 100
Separating 97 clusters into 288 cells using the watershed transform
Segmenting image 31 / 100
Separating 94 clusters into 284 cells using the watershed transform
Separating 99 clusters into 323 cells using the watershed transform
Computing features for image 38 / 100
Segmenting image 45 / 100
Separating 94 clusters into 275 cells using the watershed transform
Computing features for image 45 / 100
Segmenting image 6 / 100
Separating 97 clusters into 293 cells using the watershed transform
Computing features for image 6 / 100
Segmenting image 10 / 100
Separating 98 clusters into 280 cells using the watershed transform
Computing features for image 10 / 100
Segmenting image 16 / 100
Separating 90 clusters into 263 cells using the watershed transform
Computing features for image 16 / 100
Computing features for image 23 / 100
Computing features for image 31 / 100
Separating 97 clusters into 273 cells using the watershed transform
Computing features for image 53 / 100
Segmenting image 59 / 100
Segmenting image 30 / 100
Separating 96 clusters into 290 cells using the watershed transform
Computing features for image 30 / 100
Segmenting image 37 / 100
Separating 94 clusters into 286 cells using the watershed transform
Computing features for image 37 / 100
Segmenting image 44 / 100
Separating 97 clusters into 293 cells using the watershed transform
Computing features for image 44 / 100
Segmenting image 52 / 100
Separating 91 clusters into 283 cells using the watershed transform
Computing features for image 52 / 100
Segmenting image 9 / 100
Separating 91 clusters into 253 cells using the watershed transform
Computing features for image 9 / 100
Segmenting image 15 / 100
Separating 104 clusters into 291 cells using the watershed transform
Computing features for image 15 / 100
Segmenting image 22 / 100
Separating 95 clusters into 264 cells using the watershed transform
Computing features for image 22 / 100
Segmenting image 29 / 100
Separating 96 clusters into 296 cells using the watershed transform
Segmenting image 36 / 100
Separating 87 clusters into 276 cells using the watershed transform
Segmenting image 43 / 100
Separating 99 clusters into 286 cells using the watershed transform
Segmenting image 51 / 100
Separating 89 clusters into 275 cells using the watershed transform
Separating 104 clusters into 294 cells using the watershed transform
Computing features for image 59 / 100
Segmenting image 65 / 100
Segmenting image 21 / 100
Separating 89 clusters into 250 cells using the watershed transform
Computing features for image 29 / 100
Computing features for image 36 / 100
Computing features for image 43 / 100
Computing features for image 51 / 100
Segmenting image 58 / 100
Separating 113 clusters into 312 cells using the watershed transform
Computing features for image 58 / 100
Separating 101 clusters into 298 cells using the watershed transform
Computing features for image 65 / 100
Segmenting image 14 / 100
Separating 108 clusters into 304 cells using the watershed transform
Computing features for image 14 / 100
Computing features for image 21 / 100
Segmenting image 57 / 100
Separating 106 clusters into 304 cells using the watershed transform
Segmenting image 13 / 100
Separating 90 clusters into 247 cells using the watershed transform
Computing features for image 13 / 100
Segmenting image 20 / 100
Separating 100 clusters into 286 cells using the watershed transform
Computing features for image 20 / 100
Segmenting image 28 / 100
Separating 98 clusters into 291 cells using the watershed transform
Computing features for image 28 / 100
Segmenting image 35 / 100
Separating 97 clusters into 297 cells using the watershed transform
Computing features for image 35 / 100
Segmenting image 42 / 100
Separating 93 clusters into 276 cells using the watershed transform
Computing features for image 42 / 100
Segmenting image 50 / 100
Separating 92 clusters into 276 cells using the watershed transform
Computing features for image 50 / 100
Computing features for image 57 / 100
Segmenting image 64 / 100
Separating 100 clusters into 304 cells using the watershed transform
Computing features for image 64 / 100
Segmenting image 19 / 100
Separating 94 clusters into 266 cells using the watershed transform
Computing features for image 19 / 100
Segmenting image 27 / 100
Separating 96 clusters into 290 cells using the watershed transform
Computing features for image 27 / 100
Segmenting image 34 / 100
Separating 96 clusters into 299 cells using the watershed transform
Computing features for image 34 / 100
Segmenting image 41 / 100
Separating 93 clusters into 272 cells using the watershed transform
Computing features for image 41 / 100
Segmenting image 40 / 100
Separating 90 clusters into 272 cells using the watershed transform
Segmenting image 49 / 100
Separating 91 clusters into 271 cells using the watershed transform
Computing features for image 49 / 100
Segmenting image 56 / 100
Separating 104 clusters into 297 cells using the watershed transform
Computing features for image 56 / 100
Segmenting image 63 / 100
Separating 112 clusters into 320 cells using the watershed transform
Computing features for image 63 / 100
Segmenting image 70 / 100
Separating 96 clusters into 300 cells using the watershed transform
Computing features for image 70 / 100
Segmenting image 26 / 100
Separating 102 clusters into 286 cells using the watershed transform
Computing features for image 26 / 100
Segmenting image 33 / 100
Separating 84 clusters into 260 cells using the watershed transform
Computing features for image 33 / 100
Computing features for image 40 / 100
Segmenting image 48 / 100
Separating 95 clusters into 262 cells using the watershed transform
Computing features for image 48 / 100
Segmenting image 74 / 100
Segmenting image 78 / 100
Segmenting image 47 / 100
Separating 100 clusters into 290 cells using the watershed transform
Computing features for image 47 / 100
Segmenting image 55 / 100
Separating 98 clusters into 287 cells using the watershed transform
Computing features for image 55 / 100
Segmenting image 62 / 100
Separating 91 clusters into 267 cells using the watershed transform
Computing features for image 62 / 100
Segmenting image 69 / 100
Separating 101 clusters into 306 cells using the watershed transform
Computing features for image 69 / 100
Separating 105 clusters into 286 cells using the watershed transform
Computing features for image 74 / 100
Segmenting image 84 / 100
Segmenting image 81 / 100
Segmenting image 54 / 100
Separating 88 clusters into 261 cells using the watershed transform
Computing features for image 54 / 100
Segmenting image 61 / 100
Separating 107 clusters into 302 cells using the watershed transform
Computing features for image 61 / 100
Segmenting image 60 / 100
Separating 107 clusters into 296 cells using the watershed transform
Segmenting image 68 / 100
Separating 95 clusters into 302 cells using the watershed transform
Computing features for image 68 / 100
Segmenting image 73 / 100
Separating 111 clusters into 316 cells using the watershed transform
Separating 94 clusters into 263 cells using the watershed transform
Computing features for image 84 / 100
Separating 100 clusters into 301 cells using the watershed transform
Computing features for image 78 / 100
Segmenting image 77 / 100
Separating 103 clusters into 310 cells using the watershed transform
Separating 102 clusters into 296 cells using the watershed transform
Computing features for image 81 / 100
Segmenting image 86 / 100
Segmenting image 88 / 100
Computing features for image 60 / 100
Computing features for image 73 / 100
Segmenting image 83 / 100
Separating 97 clusters into 301 cells using the watershed transform
Computing features for image 77 / 100
Segmenting image 80 / 100
Separating 107 clusters into 285 cells using the watershed transform
Segmenting image 67 / 100
Separating 100 clusters into 285 cells using the watershed transform
Computing features for image 67 / 100
Segmenting image 72 / 100
Separating 99 clusters into 312 cells using the watershed transform
Computing features for image 72 / 100
Computing features for image 83 / 100
Segmenting image 82 / 100
Segmenting image 76 / 100
Separating 106 clusters into 318 cells using the watershed transform
Computing features for image 80 / 100
Segmenting image 79 / 100
Separating 109 clusters into 318 cells using the watershed transform
Separating 110 clusters into 311 cells using the watershed transform
Computing features for image 86 / 100
Segmenting image 85 / 100
Separating 98 clusters into 293 cells using the watershed transform
Separating 111 clusters into 298 cells using the watershed transform
Computing features for image 88 / 100
Segmenting image 90 / 100
Segmenting image 66 / 100
Separating 95 clusters into 275 cells using the watershed transform
Computing features for image 66 / 100
Separating 106 clusters into 318 cells using the watershed transform
Computing features for image 82 / 100
Computing features for image 76 / 100
Computing features for image 79 / 100
Computing features for image 85 / 100
Segmenting image 87 / 100
Separating 104 clusters into 282 cells using the watershed transform
Computing features for image 87 / 100
Separating 106 clusters into 296 cells using the watershed transform
Computing features for image 90 / 100
Segmenting image 71 / 100
Separating 99 clusters into 288 cells using the watershed transform
Computing features for image 71 / 100
Segmenting image 97 / 100
Segmenting image 95 / 100
Segmenting image 96 / 100
Segmenting image 98 / 100
Segmenting image 89 / 100
Separating 100 clusters into 287 cells using the watershed transform
Computing features for image 89 / 100
Segmenting image 92 / 100
Segmenting image 93 / 100
Segmenting image 75 / 100
Separating 93 clusters into 284 cells using the watershed transform
Computing features for image 75 / 100
Separating 94 clusters into 271 cells using the watershed transform
Computing features for image 97 / 100
Separating 100 clusters into 292 cells using the watershed transform
Computing features for image 98 / 100
Segmenting image 99 / 100
Separating 97 clusters into 284 cells using the watershed transform
Computing features for image 92 / 100
Separating 100 clusters into 294 cells using the watershed transform
Computing features for image 95 / 100
Segmenting image 94 / 100
Separating 115 clusters into 316 cells using the watershed transform
Computing features for image 96 / 100
Separating 93 clusters into 267 cells using the watershed transform
Computing features for image 93 / 100
Segmenting image 100 / 100
Separating 98 clusters into 299 cells using the watershed transform
Separating 110 clusters into 300 cells using the watershed transform
Computing features for image 99 / 100
Segmenting image 91 / 100
Separating 112 clusters into 306 cells using the watershed transform
Computing features for image 91 / 100
Separating 108 clusters into 313 cells using the watershed transform
Computing features for image 94 / 100
Computing features for image 100 / 100
Segmentation Done.

AFTER segmentation ,it seems that program is end , but i saw there's a tracking process in next.

[klasma]

My guess is that the software is busy computing migration probabilities and mitosis probabilities. This can take a long time (several hours) if you have a lot (thousands) of segmented objects in each frame. Unfortunately, there are no prinouts in the methods which compute probabilites. That is something I will add in the future. Try leaving the software overnight to see if it gets to the track linking. If that does not work, you can try pressing the pause button in Matlab to see what the program is doing.

The following information would be useful:

What operating system do you have?
Are you running the program in Matlab or are you running the standalone version?
For how long did you wait?
How many objects do you have in each frame?

[klasma]

You should also take a look at how much RAM the program is using. The run time will be shorter and you will need less RAM i you do not allow cell division, by setting pSplit to 0 in the tracking settings. You can try that if waiting is not enough.