diff --git a/docs/usage.md b/docs/usage.md
index 31a0966fe..33ee55f6e 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -6,7 +6,7 @@
 
 ## Samplesheet input
 
-You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 5 columns, and a header row as shown in the examples below.
+You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 7 columns, and a header row as shown in the examples below.
 
 ```bash
 --input '[path to samplesheet file]'