Problem with FACs filtering

[j-solor]

Hello,

After trying to use your code to replicate the figures as pictured in this repository, once loaded and fitted the available data in https://figshare.com/articles/dataset/methyldeconv_data/28563854/2 I've reached a point where only the Bcells and the Macrophages seem to have an estimation that matches the original. It seems that there could be a problem either with the ground truth preparation, either on the FACs file or the proccessing of it.

I believe it could have to do with the "CD8+, CD4+" when generating "facs_df" in the 208-250

Aside, NKcells could have a difference too

This is very noticeable with the scatterplot and heatmap.

Thanks in advance

[alex-d13]

Hi,

This was indeed an issue with FigShare, I was not aware that it creates ';' as ','. Then it split the rows with 'CD8+, CD4+' into two cells.
Version4 of deconvMe data has this fixed now, I also updated the scripts in this repository.

Thank you for bringing it up, please let me know if you can reproduce the results now!

Best,
Alex