Tecan Infinite 200 PRO plate reader backend (Infinite M Plex)

docs/user_guide/02_analytical/plate-reading/plate-reading.ipynb

@@ -4,21 +4,7 @@
    "cell_type": "markdown",
    "id": "39d0c1a5",
    "metadata": {},
-   "source": [
-    "# Plate reading\n",
-    "\n",
-    "PyLabRobot supports the following plate readers:\n",
-    "\n",
-    "```{toctree}\n",
-    ":maxdepth: 1\n",
-    "\n",
-    "bmg-clariostar\n",
-    "cytation\n",
-    "synergyh1\n",
-    "```\n",
-    "\n",
-    "This example uses the `PlateReaderChatterboxBackend`. When using a real machine, use the corresponding backend."
-   ]
+   "source": "# Plate reading\n\nPyLabRobot supports the following plate readers:\n\n```{toctree}\n:maxdepth: 1\n\nbmg-clariostar\ncytation\nsynergyh1\ntecan-infinite\n```\n\nThis example uses the `PlateReaderChatterboxBackend`. When using a real machine, use the corresponding backend."
   },
   {
    "cell_type": "code",
@@ -432,4 +418,4 @@
  },
  "nbformat": 4,
  "nbformat_minor": 5
-}
+}

docs/user_guide/02_analytical/plate-reading/tecan-infinite.ipynb

@@ -0,0 +1,186 @@
+{
+ "cells": [
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "# Tecan Infinite 200 PRO\n",
+    "\n",
+    "The Tecan Infinite 200 PRO is a multimode microplate reader that supports absorbance, fluorescence, and luminescence measurements. This backend targets the Infinite \"M\" series (e.g., Infinite 200 PRO M Plex)."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "from pylabrobot.plate_reading import PlateReader\n",
+    "from pylabrobot.plate_reading.tecan import TecanInfinite200ProBackend"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "pr = PlateReader(name=\"PR\", size_x=0, size_y=0, size_z=0, backend=TecanInfinite200ProBackend())\n",
+    "await pr.setup()"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "await pr.open()"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "Before closing, assign a plate to the plate reader. This determines the well positions for measurements."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "from pylabrobot.resources import Cor_96_wellplate_360ul_Fb\n",
+    "plate = Cor_96_wellplate_360ul_Fb(name=\"plate\")\n",
+    "pr.assign_child_resource(plate)"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "await pr.close()"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## Absorbance\n",
+    "\n",
+    "Read absorbance at a specified wavelength (230-1000 nm)."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "import matplotlib.pyplot as plt\n",
+    "\n",
+    "data = await pr.read_absorbance(wavelength=450)\n",
+    "plt.imshow(data)\n",
+    "plt.colorbar(label=\"OD\")\n",
+    "plt.title(\"Absorbance at 450 nm\")"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## Fluorescence\n",
+    "\n",
+    "Read fluorescence with specified excitation and emission wavelengths (230-850 nm) and focal height."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "data = await pr.read_fluorescence(\n",
+    "    excitation_wavelength=485,\n",
+    "    emission_wavelength=528,\n",
+    "    focal_height=7.5\n",
+    ")\n",
+    "plt.imshow(data)\n",
+    "plt.colorbar(label=\"RFU\")\n",
+    "plt.title(\"Fluorescence (Ex: 485 nm, Em: 528 nm)\")"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## Luminescence\n",
+    "\n",
+    "Read luminescence with a specified focal height."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "data = await pr.read_luminescence(focal_height=4.5)\n",
+    "plt.imshow(data)\n",
+    "plt.colorbar(label=\"RLU\")\n",
+    "plt.title(\"Luminescence\")"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## Reading specific wells\n",
+    "\n",
+    "You can specify a subset of wells to read instead of the entire plate."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "wells = plate.get_items([\"A1\", \"A2\", \"B1\", \"B2\"])\n",
+    "data = await pr.read_absorbance(wavelength=450, wells=wells)"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## Cleanup"
+   ]
+  },
+  {
+   "cell_type": "code",
+   "execution_count": null,
+   "metadata": {},
+   "outputs": [],
+   "source": [
+    "await pr.stop()"
+   ]
+  }
+ ],
+ "metadata": {
+  "kernelspec": {
+   "display_name": "Python 3",
+   "language": "python",
+   "name": "python3"
+  },
+  "language_info": {
+   "name": "python",
+   "version": "3.10.0"
+  }
+ },
+ "nbformat": 4,
+ "nbformat_minor": 4
+}

docs/user_guide/machines.md

@@ -159,6 +159,7 @@ tr > td:nth-child(5) { width: 15%; }
 | Byonoy | Luminescence 96 Automate | <span class="badge badge-luminescence">luminescence</span> | WIP | [OEM](https://byonoy.com/luminescence-96-automate/) |
 | Molecular Devices | SpectraMax M5e | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span> <span class="badge badge-time-resolved-fluo">time-resolved fluorescence</span><span class="badge badge-fluo-polarization">fluorescence polarization</span> | Full | [OEM](https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers) |
 | Molecular Devices | SpectraMax 384plus | <span class="badge badge-absorbance">absorbance</span> | Full | [OEM](https://www.moleculardevices.com/products/microplate-readers/absorbance-readers/spectramax-abs-plate-readers) |
+| Tecan | Infinite 200 PRO | <span class="badge badge-absorbance">absorbance</span><span class="badge badge-fluorescence">fluorescence</span><span class="badge badge-luminescence">luminescence</span> | Mostly | [PLR](https://docs.pylabrobot.org/user_guide/02_analytical/plate-reading/tecan-infinite.html) / [OEM](https://lifesciences.tecan.com/infinite-200-pro) |
 
 
 ### Flow Cytometers

pylabrobot/plate_reading/__init__.py

@@ -44,3 +44,4 @@
   ImagingResult,
   Objective,
 )
+from .tecan import InfiniteScanConfig, TecanInfinite200ProBackend

pylabrobot/plate_reading/tecan/__init__.py

@@ -0,0 +1 @@
+from .infinite_backend import InfiniteScanConfig, TecanInfinite200ProBackend