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This repo contains the data from: EVCouplings, DynaMut2, MDM-TASK, MD simulation. These files are important for understanding book chapter 17 "Computational Methods to Engineer Cas Proteins for Efficient Genome Editing"

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Cas9_Files

This repo contains the data from: EVCouplings, DynaMut2, MDM-TASK, MD simulation.

MD Simulation:

For PBC correction (1 Protein and 0 System)

echo 1, 0 |gmx trjconv -s md.tpr -f md.xtc -o md_corr.xtc -center -ur compact -pbc mol

Visualization in VMD

PBC Correction:

vmd step3_input.psf md_corr.xtc

Protein Alignment:

VMD Main > Extension > Analysis > RMSD Trajectory Tool > Align (using protein) > RMSD

VMD Main > Graphics > Representations > index 0 to 26513 (in Selection Atoms Panel) > Enter

Save Protein-Nucleic Acid Complex in .dcd format:

VMD Main > File > Save Coordinates > Selected Atoms: index 0 to 26513 > File Type: dcd > Save > Protein_NA_complex.dcd

VMD Main > File > Save Coordinates > Selected Atoms: index 0 to 26513 > File Type: pdb > Frames: First 0, Last 0, Stride, 1 > Save > Protein_NA_complex.pdb

Visualizing Protein-Nucleic Acid Complex:

vmd Protein_NA_complex.pdb Protein_NA_complex.dcd

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This repo contains the data from: EVCouplings, DynaMut2, MDM-TASK, MD simulation. These files are important for understanding book chapter 17 "Computational Methods to Engineer Cas Proteins for Efficient Genome Editing"

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