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Protocols used by AOML Omics labs, following the BeBOP-OBON standard
Water collection and filtration protocol using sterivex filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Water collection and filtration protocol using Smith-Root self preservng eDNA filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
Water collection + EtOH subsampling protocol, plus subsequent filtration using disc filters and a vacuum manifold. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.
This PCR protocol is for amplifying the mitochondrial displacement loop (D-loop) gene also known as the mitochondial control region in marine mammals.
Protocol for preparing COI (Leray-Geller) amplicon libraries at Smithsonian NMNH
This PCR protocol is for amplifying the Small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v9 gene in eukaryotes.
This protocol is for running and imaging PCR product using gel electrophoresis.
Protocol for running the Tourmaline 2 amplicon sequence processing workflow on data from assay Eukarya-18S-V9-Lane-Medlin
This PCR protocol is for amplifying the 16S rRNA v4-v5 region.
This PCR protocol is for amplifying the 16S large subunit ribosomal RNA mitochondrial gene in eukaryotes.
Protocol for preparing metagenomic libraries using Illumina DNA Prep
Protocol for preparing 18S V9 (EMP) amplicon libraries for Michigan State RTSF
Protocol for sampling water using Sterivex with no ZR beads or preservative (dry)
Protocol for extraction of DNA from Sterivex filters using a KingFisher robot (BeBOP format)
Organismal subsampling and Nucleic acids extraction from the tissue samples using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These collection and extraction protocols used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This PCR protocol is for amplifying the 12S mitochondrial ribosomal RNA gene in vertebrates.
Protocol for preparing 12S V5-V6 (MiFish) amplicon libraries for Michigan State RTSF
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